A Na‐dependent fructose co‐transporter in lobster hepatopancreas

Identification of a novel sodium-dependent fructose transport activity in the hepatopancreas of the Atlantic lobster Homarus americanus.

[(3)H]Fructose and [(3)H]glucose transport were determined in brush-border membrane vesicles (BBMV), basolateral membrane vesicles (BLMV) and isolated cells (E, R, F, B) of H. americanus (Atlantic lobster) hepatopancreas. Glucose transport in BBMV was equilibrative in the absence of sodium and concentrative in the presence of sodium. Sodium-dependent glucose transport by BBMV was not inhibited ...

Dual control of cytosolic metals by lysosomal transporters in lobster hepatopancreas.

This study describes the membrane transport mechanisms used by lobster (Homarus americanus) hepatopancreatic epithelial lysosomes to accumulate and sequester heavy metals from the cytosol, and thereby aid in the regulation of these ions entering the animal from dietary constituents. The present investigation extends previous work describing lysosomal metal uptake by cation exchange with protons...

The oxalate/sulfate antiporter in lobster hepatopancreas: internal and external binding constants.

Utilizing a purified basolateral plasma membrane vesicle (BLMV) preparation containing a sulfate/oxalate antiporter, it was demonstrated that sulfate exhibited similar binding characteristics to the transporter whether bound internally or externally. It was also demonstrated that oxalate had similar binding characteristics to the antiporter whether it was bound internally or externally. Oxalate...

Cytosolic Distribution of Cd2+, Cu2+, and Zn2+ Among Metallothionein-Like Proteins in Lobster Hepatopancreas

Zinc and Cu metabolism in crustacea changes dramatically during the molt cycle. Metallothionein (MT) and MT-like proteins are implicated in the handling of both metal ions as well as in the detoxification of Cd. Distribution of Cd2 / Cu / and Zn2 / in cytosol and among MT-like species has been determined. Lobsters were treated to induce MT as in mammals with dexamethasone (dex) or stress from t...

Transepithelial D-glucose and D-fructose Transport Across Lobster Intestine